A Laboratory Technician in a biotech company performs precise experiments and tests to support research and development of new products. They handle sample preparation, operate advanced laboratory equipment, and maintain accurate records to ensure reliable data. Their role is crucial in ensuring quality control and assisting scientists in innovative biotechnological discoveries.
Sample preparation and processing
Sample preparation and processing involves accurately handling and treating specimens to ensure reliable and consistent results in laboratory testing. Key tasks include measuring, mixing, and preserving samples according to standardized protocols, while maintaining strict adherence to safety and quality control guidelines. Mastery of sample preparation techniques directly impacts the validity of experimental outcomes and diagnostic accuracy.
DNA/RNA extraction and purification
Expertise in DNA/RNA extraction and purification is essential for accurate molecular biology analyses, requiring proficiency with techniques such as spin column purification, magnetic bead-based extraction, and organic solvent methods. Candidates should demonstrate knowledge of sample handling to prevent nucleic acid degradation, as well as experience with quantification and quality assessment using spectrophotometry and electrophoresis. Familiarity with laboratory best practices, contamination control, and maintaining detailed documentation enhances reproducibility and reliability in genomic studies.
Performing PCR and qPCR assays
Performing PCR and qPCR assays involves accurately preparing samples, optimizing reaction conditions, and analyzing amplification results to quantify DNA or RNA targets. Candidates should have experience with laboratory protocols, data interpretation software, and maintaining meticulous records for reproducibility. Proficiency in troubleshooting assay performance and ensuring quality control standards is essential for reliable molecular diagnostics.
Gel electrophoresis and imaging
Expertise in gel electrophoresis techniques is essential for separating DNA, RNA, or proteins based on molecular size and charge. Proficiency in imaging systems such as UV transilluminators and gel documentation units ensures accurate visualization and analysis of electrophoresis results. Experience with buffer preparation, gel casting, and troubleshooting common electrophoresis issues is highly valued for maintaining experiment reliability and data quality.
Cell culture maintenance
Proficient in cell culture maintenance, ensuring optimal growth conditions by monitoring media quality, controlling contamination, and maintaining sterile techniques. Experienced in routine passaging, cryopreservation, and accurate record-keeping to support consistent experimental results. Skilled in troubleshooting culture issues and implementing protocols to enhance cell viability and productivity.
Buffer and reagent preparation
Prepare and accurately measure buffers and reagents following standardized protocols to ensure experimental consistency and reliability. Maintain detailed records of preparation processes, concentrations, and batch numbers to support quality control. Use high-purity chemicals and calibrated equipment to optimize the buffer and reagent preparation process, minimizing contamination and errors.
Data collection and documentation
Collect, organize, and maintain accurate data records to support analysis and reporting processes. Ensure thorough documentation of methodologies and data sources to enhance transparency and reproducibility. Strong attention to detail and proficiency with data management tools are critical for effective data collection and documentation.
Laboratory equipment calibration and maintenance
Perform routine laboratory equipment calibration to ensure accuracy and reliability of test results; maintain detailed logs of calibration activities and equipment performance. Troubleshoot and repair laboratory instruments, adhering to manufacturer specifications and safety standards to minimize downtime. Collaborate with quality control teams to implement preventive maintenance schedules and improve laboratory operational efficiency.
Sterilization and inventory management
Expertise in sterilization procedures ensures all medical instruments and equipment are thoroughly disinfected and safe for use, adhering to stringent healthcare standards. Proficient in inventory management involves tracking supplies, coordinating reorders, and maintaining accurate records to prevent shortages or overstock. Recommend implementing digital inventory systems to enhance efficiency and reduce human error in stock control.
Assisting with experimental protocol development
Supporting the design and refinement of experimental protocols to ensure accurate data collection and reproducibility. Collaborating with research teams to align procedures with project objectives and compliance standards. Providing input on best practices and troubleshooting protocol challenges to optimize experimental outcomes.